3F6P

Crystal Structure of unphosphorelated receiver domain of YycF

Summary for 3F6P

• Entry DOI: 10.2210/pdb3f6p/pdb
• Descriptor: Transcriptional regulatory protein yycF (2 entities in total)
• Functional Keywords: unphosphorelated, receiver domain, cytoplasm, dna-binding, phosphoprotein, transcription, transcription regulation, two-component regulatory system, dna binding protein, transcription regulator
• Biological source: Bacillus subtilis
• Cellular location: Cytoplasm : P37478
• Total number of polymer chains: 1
• Total formula weight: 13787.98

Authors

Zhao, H.,Tang, L. (deposition date: 2008-11-06, release date: 2010-03-02, Last modification date: 2023-09-06)

Primary citation

Zhao, H.,Heroux, A.,Sequeira, R.D.,Tang, L.
Preliminary crystallographic studies of the regulatory domain of response regulator YycF from an essential two-component signal transduction system.
Acta Crystallogr.,Sect.F, 65:719-722, 2009

PubMed Abstract: YycGF is a crucial signal transduction system for the regulation of cell-wall metabolism in low-G+C Gram-positive bacteria, which include many important human pathogens. The response regulator YycF receives signals from its cognate histidine kinase YycG through a phosphotransfer reaction and elicits responses through regulation of gene expression. The N-terminal regulatory domain of YycF from Bacillus subtilis was overproduced and purified. The protein was crystallized and X-ray data were collected to 1.95 A resolution with a completeness of 97.7% and an overall R(merge) of 7.7%. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 59.50, c = 79.06 A.

PubMed: 19574649
DOI: 10.1107/S1744309109022696

Experimental method

X-RAY DIFFRACTION (1.95 Å)