3F3T

Kinase domain of cSrc in complex with inhibitor RL38 (Type III)

3F3T の概要

• エントリーDOI: 10.2210/pdb3f3t/pdb
• 関連するPDBエントリー: 2QI8 2QLQ 2QQ7 3F3U 3F3V 3F3W
• 分子名称: Proto-oncogene tyrosine-protein kinase Src, 1-[1-(3-aminophenyl)-3-tert-butyl-1H-pyrazol-5-yl]-3-naphthalen-1-ylurea (3 entities in total)
• 機能のキーワード: allosteric, type iii, dfg-out, alternative splicing, atp-binding, kinase, lipoprotein, myristate, nucleotide-binding, phosphoprotein, proto-oncogene, sh2 domain, sh3 domain, transferase, tyrosine-protein kinase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Cell membrane (By similarity): P00523
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66284.40

構造登録者

Gruetter, C.,Klueter, S.,Getlik, M.,Rauh, D. (登録日: 2008-10-31, 公開日: 2009-03-03, 最終更新日: 2025-05-28)

主引用文献

Simard, J.R.,Kluter, S.,Grutter, C.,Getlik, M.,Rabiller, M.,Rode, H.B.,Rauh, D.
A new screening assay for allosteric inhibitors of cSrc
Nat.Chem.Biol., 5:394-396, 2009

PubMed Abstract: Targeting kinases outside the highly conserved ATP pocket is thought to be a promising strategy for overcoming bottlenecks in kinase inhibitor research, such as limited selectivity and drug resistance. Here we report the development and application of a direct binding assay to detect small molecules that stabilize the inactive conformation of the tyrosine kinase cSrc. Protein X-ray crystallography validated the assay results and confirmed an exclusively allosteric binding mode.

PubMed: 19396179
DOI: 10.1038/nchembio.162

実験手法

X-RAY DIFFRACTION (2.5 Å)