3EW5

Structure of the tetragonal crystal form of X (ADRP) domain from FCoV

Summary for 3EW5

• Entry DOI: 10.2210/pdb3ew5/pdb
• Related: 3eti
• Descriptor: macro domain of Non-structural protein 3, CHLORIDE ION, SULFATE ION, ... (5 entities in total)
• Functional Keywords: coronavirus, macro, adrp, x domain, rna binding protein
• Biological source: Feline infectious peritonitis virus (FCoV)
• Cellular location: Non-structural protein 3: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 4: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 6: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 7: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 9: Host cytoplasm, host perinuclear region (By similarity). Non-structural protein 10: Host cytoplasm, host perinuclear region (By similarity). Helicase: Host endoplasmic reticulum-Golgi intermediate compartment (Potential). Uridylate-specific endoribonuclease: Host cytoplasm, host perinuclear region (By similarity): Q98VG9
• Total number of polymer chains: 3
• Total formula weight: 56552.33

Authors

Wojdyla, J.A.,Manolaridis, I.,Tucker, P.A. (deposition date: 2008-10-14, release date: 2009-10-27, Last modification date: 2023-12-27)

Primary citation

Wojdyla, J.A.,Manolaridis, I.,Snijder, E.J.,Gorbalenya, A.E.,Coutard, B.,Piotrowski, Y.,Hilgenfeld, R.,Tucker, P.A.
Structure of the X (ADRP) domain of nsp3 from feline coronavirus
Acta Crystallogr.,Sect.D, 65:1292-1300, 2009

PubMed Abstract: The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

PubMed: 19966415
DOI: 10.1107/S0907444909040074

Experimental method

X-RAY DIFFRACTION (3.1 Å)