3EVB

Crystal structure of yellow fever virus methyltransferase complexed with S-adenosyl-L-homocysteine

Summary for 3EVB

• Entry DOI: 10.2210/pdb3evb/pdb
• Related: 3EVA 3EVC 3EVD 3EVE 3EVF 3EVG
• Descriptor: RNA-directed RNA polymerase NS5, S-ADENOSYL-L-HOMOCYSTEINE (3 entities in total)
• Functional Keywords: yellow fever virus, ns5 methyltransferase, rna cap binding, atp-binding, capsid protein, cleavage on pair of basic residues, endoplasmic reticulum, envelope protein, glycoprotein, helicase, hydrolase, membrane, metal-binding, multifunctional enzyme, nucleotide-binding, nucleotidyltransferase, nucleus, phosphoprotein, protease, ribonucleoprotein, rna replication, rna-binding, rna-directed rna polymerase, secreted, serine protease, transmembrane, viral nucleoprotein, virion, transferase
• Biological source: Yellow fever virus
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted. Small envelope protein M: Virion membrane; Multi-pass membrane protein (Potential). Envelope protein E: Virion membrane; Multi- pass membrane protein (Potential). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P03314
• Total number of polymer chains: 1
• Total formula weight: 31675.22

Authors

Thompson, A.A.,Geiss, B.J.,Peersen, O.B. (deposition date: 2008-10-13, release date: 2009-01-06, Last modification date: 2023-09-06)

• Primary citation: Geiss, B.J.,Thompson, A.A.,Andrews, A.J.,Sons, R.L.,Gari, H.H.,Keenan, S.M.,Peersen, O.B.; Analysis of flavivirus NS5 methyltransferase cap binding.; J.Mol.Biol., 385:1643-1654, 2009; PubMed: 19101564; DOI: 10.1016/j.jmb.2008.11.058

Experimental method

X-RAY DIFFRACTION (1.85 Å)