3EU7

Crystal Structure of a PALB2 / BRCA2 complex

Summary for 3EU7

• Entry DOI: 10.2210/pdb3eu7/pdb
• Descriptor: Partner and localizer of BRCA2, 19meric peptide from Breast cancer type 2 susceptibility protein, GLYCEROL, ... (4 entities in total)
• Functional Keywords: wd40 domain, beta propeller, protein-peptide complex, fanconi anemia, nucleus, phosphoprotein, wd repeat, disease mutation, dna damage, dna repair, transcription-antitumor protein complex, transcription/antitumor protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q86YC2 P51587
• Total number of polymer chains: 2
• Total formula weight: 41487.28

Authors

Oliver, A.W.,Pearl, L.H. (deposition date: 2008-10-09, release date: 2009-07-28, Last modification date: 2024-10-16)

Primary citation

Oliver, A.W.,Swift, S.,Lord, C.J.,Ashworth, A.,Pearl, L.H.
Structural basis for recruitment of BRCA2 by PALB2
Embo Rep., 10:990-996, 2009

PubMed Abstract: The breast cancer 2, early onset protein (BRCA2) is central to the repair of DNA damage by homologous recombination. BRCA2 recruits the recombinase RAD51 to sites of damage, regulates its assembly into nucleoprotein filaments and thereby promotes homologous recombination. Localization of BRCA2 to nuclear foci requires its association with the partner and localizer of BRCA2 (PALB2), mutations in which are associated with cancer predisposition, as well as subtype N of Fanconi anaemia. We have determined the structure of the PALB2 carboxy-terminal beta-propeller domain in complex with a BRCA2 peptide. The structure shows the molecular determinants of this important protein-protein interaction and explains the effects of both cancer-associated truncating mutants in PALB2 and missense mutations in the amino-terminal region of BRCA2.

PubMed: 19609323
DOI: 10.1038/embor.2009.126

Experimental method

X-RAY DIFFRACTION (2.2 Å)