3EOX

High quality structure of cleaved PAI-1-stab

Summary for 3EOX

Entry DOI	10.2210/pdb3eox/pdb
Related	1DB2 9PAI
Descriptor	Plasminogen activator inhibitor 1 (2 entities in total)
Functional Keywords	cleaved plasminogen activator inhibitor-1, pai-1, serpin, stabilized, glycoprotein, plasminogen activation, protease inhibitor, secreted, serine protease inhibitor, blood clotting
Biological source	Homo sapiens (Human)
Cellular location	Secreted: P05121
Total number of polymer chains	1
Total formula weight	42751.01
Authors	Dewilde, M.,Declerck, P.J.,Rabijns, A.,Strelkov, S.V. (deposition date: 2008-09-29, release date: 2009-02-17, Last modification date: 2023-11-01)
Primary citation	Dewilde, M.,Strelkov, S.V.,Rabijns, A.,Declerck, P.J. High quality structure of cleaved PAI-1-stab. J.Struct.Biol., 165:126-132, 2009 Cited by PubMed Abstract: Here we report the crystal structure of a stablilized plasminogen activator inhibitor-1 variant (PAI-1-N150H-K154T-Q301P-Q319L-M354I (PAI-1-stab)) that shows a cleavage within the reactive centre loop. The new structure is of superior quality compared to the previously determined structure of the cleaved PAI-1-A335P mutant. We present a detailed comparison of the two structures and also compare them with the structure of the active PAI-1-stab. The structural data give important insights into the working mechanism of PAI-1 and also explain the role of various stabilizing mutations. PubMed: 19059484 DOI: 10.1016/j.jsb.2008.11.001 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.61 Å)

Structure validation