3EOX
High quality structure of cleaved PAI-1-stab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8084 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 71.174, 71.174, 141.664 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 2.610 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9pai |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.792 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.730 |
| High resolution limit [Å] | 2.610 |
| Number of reflections | 12280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.5M lithium sulphate monohydrate, 15% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
