3ENJ
Structure of Pig Heart Citrate Synthase at 1.78 A resolution
Summary for 3ENJ
| Entry DOI | 10.2210/pdb3enj/pdb |
| Related | 1CTS 2CTS 4CTS |
| Descriptor | Citrate synthase, CYSTEINE, CHLORIDE ION, ... (5 entities in total) |
| Functional Keywords | disulfide exchange, structural genomics, psi-2, protein structure initiative, center for high-throughput structural biology, chtsb, methylation, mitochondrion, transferase, transit peptide, tricarboxylic acid cycle, lyase |
| Biological source | Sus scrofa (pig,swine,wild boar) |
| Total number of polymer chains | 1 |
| Total formula weight | 49229.73 |
| Authors | Larson, S.B.,Day, J.S.,Nguyen, C.,Cudney, R.,McPherson, A.,Center for High-Throughput Structural Biology (CHTSB) (deposition date: 2008-09-25, release date: 2009-02-03, Last modification date: 2024-11-06) |
| Primary citation | Larson, S.B.,Day, J.S.,Nguyen, C.,Cudney, R.,McPherson, A. Structure of pig heart citrate synthase at 1.78 A resolution. Acta Crystallogr.,Sect.F, 65:430-434, 2009 Cited by PubMed Abstract: Pig heart citrate synthase was crystallized from a small-molecule cocktail containing cystamine dihydrochloride, aspartame and benzamidine hydrochloride. The structure was refined to an R factor of 0.179 (R(free) = 0.222) using synchrotron data to a resolution of 1.78 A. The model includes the full-length protein, a chloride ion, a sulfate ion, 305 water molecules and an unexpected moiety attached through a disulfide linkage to Cys184, which was modeled as a half-cystamine molecule generated by disulfide exchange with the cystamine in the small-molecule cocktail. PubMed: 19407370DOI: 10.1107/S1744309109008343 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.78 Å) |
Structure validation
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