3EGD

Crystal structure of the mammalian COPII-coat protein Sec23a/24a complexed with the SNARE protein Sec22 and bound to the transport signal sequence of vesicular stomatitis virus glycoprotein

3EGD の概要

• エントリーDOI: 10.2210/pdb3egd/pdb
• 関連するPDBエントリー: 3EFO 3EG9
• 分子名称: Protein transport protein Sec23A, Protein transport protein Sec24A, Vesicle-trafficking protein SEC22b, ... (6 entities in total)
• 機能のキーワード: copii coat, transport signal sequence, vesicle, disease mutation, endoplasmic reticulum, er-golgi transport, golgi apparatus, membrane, protein transport, transport, phosphoprotein, transmembrane
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Smooth endoplasmic reticulum membrane; Peripheral membrane protein: Q15436; Cytoplasm (By similarity): O95486; Endoplasmic reticulum membrane; Single-pass type IV membrane protein (By similarity): O75396
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 189961.13

構造登録者

Goldberg, J.,Mancias, J.D. (登録日: 2008-09-10, 公開日: 2008-10-21, 最終更新日: 2024-02-21)

主引用文献

Mancias, J.D.,Goldberg, J.
Structural basis of cargo membrane protein discrimination by the human COPII coat machinery.
Embo J., 27:2918-2928, 2008

PubMed Abstract: Genomic analysis shows that the increased complexity of trafficking pathways in mammalian cells involves an expansion of the number of SNARE, Rab and COP proteins. Thus, the human genome encodes four forms of Sec24, the cargo selection subunit of the COPII vesicular coat, and this is proposed to increase the range of cargo accommodated by human COPII-coated vesicles. In this study, we combined X-ray crystallographic and biochemical analysis with functional assays of cargo packaging into COPII vesicles to establish molecular mechanisms for cargo discrimination by human Sec24 subunits. A conserved IxM packaging signal binds in a surface groove of Sec24c and Sec24d, but the groove is occluded in the Sec24a and Sec24b subunits. Conversely, LxxLE class transport signals and the DxE signal of VSV glycoprotein are selectively bound by Sec24a and Sec24b subunits. A comparative analysis of crystal structures of the four human Sec24 isoforms establishes the structural determinants for discrimination among these transport signals, and provides a framework to understand how an expansion of coat subunits extends the range of cargo proteins packaged into COPII-coated vesicles.

PubMed: 18843296
DOI: 10.1038/emboj.2008.208

実験手法

X-RAY DIFFRACTION (2.7 Å)