3EBC

Structure of N141A HincII with Cognate DNA

3EBC の概要

• エントリーDOI: 10.2210/pdb3ebc/pdb
• 分子名称: Type-2 restriction enzyme HincII, 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3', 5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3', ... (5 entities in total)
• 機能のキーワード: intermediate, mutant, dimerization, r-loop, endonuclease, hydrolase, nuclease, restriction system, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 81807.46

構造登録者

Little, E.J.,Babic, A.C.,Horton, N.C. (登録日: 2008-08-27, 公開日: 2008-12-23, 最終更新日: 2024-02-21)

主引用文献

Little, E.J.,Babic, A.C.,Horton, N.C.
Early Interrogation and Recognition of DNA Sequence by Indirect Readout
Structure, 16:1828-1837, 2008

PubMed Abstract: Control of replication, transcription, recombination and repair requires proteins capable of finding particular DNA sequences in a background of a large excess of nonspecific sequences. Such recognition can involve direct readout, with direct contacts to the bases of DNA, or in some cases through the less well-characterized indirect readout mechanisms. In order to measure the relative contributions of direct and indirect readout by a sequence specific endonuclease, HincII, a mutant enzyme deficient in a direct contact, was characterized, and surprisingly showed no loss of sequence specificity. The three dimensional crystal structure shows the loss of most of the direct readout contacts to the DNA, possibly capturing an early stage in target site recognition using predominately indirect readout to prescreen sites before full sequence interrogation.

PubMed: 19081059

実験手法

X-RAY DIFFRACTION (2.55 Å)