3E73

Crystal Structure of Human LanCL1 complexed with GSH

3E73 の概要

• エントリーDOI: 10.2210/pdb3e73/pdb
• 関連するPDBエントリー: 3E6U
• 分子名称: LanC-like protein 1, ZINC ION, GLUTATHIONE, ... (4 entities in total)
• 機能のキーワード: alpha helix barrel, cytoplasm, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: O43813
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94514.71

構造登録者

Zhang, W.,Zhu, G.,Li, X.,Rao, Z.,Zhang, C. (登録日: 2008-08-17, 公開日: 2009-06-30, 最終更新日: 2023-11-01)

主引用文献

Zhang, W.,Wang, L.,Liu, Y.,Xu, J.,Zhu, G.,Cang, H.,Li, X.,Bartlam, M.,Hensley, K.,Li, G.,Rao, Z.,Zhang, X.C.
Structure of human lanthionine synthetase C-like protein 1 and its interaction with Eps8 and glutathione
Genes Dev., 23:1387-1392, 2009

PubMed Abstract: Eukaryotic lanthionine synthetase C-like protein 1 (LanCL1) is homologous to prokaryotic lanthionine cyclases, yet its biochemical functions remain elusive. We report the crystal structures of human LanCL1, both free of and complexed with glutathione, revealing glutathione binding to a zinc ion at the putative active site formed by conserved GxxG motifs. We also demonstrate by in vitro affinity analysis that LanCL1 binds specifically to the SH3 domain of a signaling protein, Eps8. Importantly, expression of LanCL1 mutants defective in Eps8 interaction inhibits nerve growth factor (NGF)-induced neurite outgrowth, providing evidence for the biological significance of this novel interaction in cellular signaling and differentiation.

PubMed: 19528316
DOI: 10.1101/gad.1789209

実験手法

X-RAY DIFFRACTION (2.8 Å)