3E0G
Structure of the Leukemia Inhibitory Factor Receptor (LIF-R) domains D1-D5
Summary for 3E0G
| Entry DOI | 10.2210/pdb3e0g/pdb |
| Descriptor | Leukemia inhibitory factor receptor, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | ig domain, cytokine binding homology region (chr), cell membrane, disease mutation, glycoprotein, membrane, receptor, secreted, transmembrane, signaling protein-cytokine complex, signaling protein/cytokine |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 57067.00 |
| Authors | Lupardus, P.J.,Garcia, K.C. (deposition date: 2008-07-31, release date: 2008-09-23, Last modification date: 2024-10-30) |
| Primary citation | Skiniotis, G.,Lupardus, P.J.,Martick, M.,Walz, T.,Garcia, K.C. Structural organization of a full-length gp130/LIF-R cytokine receptor transmembrane complex. Mol.Cell, 31:737-748, 2008 Cited by PubMed Abstract: gp130 is a shared receptor for at least nine cytokines and can signal either as a homodimer or as a heterodimer with Leukemia Inhibitory Factor Receptor (LIF-R). Here, we biophysically and structurally characterize the full-length, transmembrane form of a quaternary cytokine receptor complex consisting of gp130, LIF-R, the cytokine Ciliary Neurotrophic Factor (CNTF), and its alpha receptor (CNTF-Ralpha). Thermodynamic analysis indicates that, unlike the cooperative assembly of the symmetric gp130/Interleukin-6/IL-6Ralpha hexameric complex, CNTF/CNTF-Ralpha heterodimerizes gp130 and LIF-R via noncooperative energetics to form an asymmetric 1:1:1:1 complex. Single particle electron microscopic analysis of the full-length gp130/LIF-R/CNTF-Ralpha/CNTF quaternary complex elucidates an asymmetric structural arrangement, in which the receptor extracellular and transmembrane segments join as a continuous, rigid unit, poised to sensitively transduce ligand engagement to the membrane-proximal intracellular signaling regions. These studies also enumerate the organizing principles for assembly of the "tall" class of gp130 family cytokine receptor complexes including LIF, IL-27, IL-12, and others. PubMed: 18775332DOI: 10.1016/j.molcel.2008.08.011 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
Download full validation report
