3E0D

Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit

3E0D の概要

• エントリーDOI: 10.2210/pdb3e0d/pdb
• 分子名称: DNA substrate template strand, DNA substrate primer strand, DNA polymerase III subunit alpha, ... (5 entities in total)
• 機能のキーワード: ob fold, nucleotidyl transferase, polymerase, beta barrel, cytoplasm, dna replication, dna-directed dna polymerase, nucleotidyltransferase, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Thermus aquaticus
• 細胞内の位置: Cytoplasm (By similarity): Q9XDH5
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 305688.24

構造登録者

Wing, R.A.,Bailey, S.,Steitz, T.A. (登録日: 2008-07-31, 公開日: 2008-09-23, 最終更新日: 2024-02-21)

主引用文献

Wing, R.A.,Bailey, S.,Steitz, T.A.
Insights into the Replisome from the Structure of a Ternary Complex of the DNA Polymerase III alpha-Subunit.
J.Mol.Biol., 382:859-869, 2008

PubMed Abstract: The crystal structure of the catalytic alpha-subunit of the DNA polymerase III (Pol IIIalpha) holoenzyme bound to primer-template DNA and an incoming deoxy-nucleoside 5'-triphosphate has been determined at 4.6-A resolution. The polymerase interacts with the sugar-phosphate backbone of the DNA across its minor groove, which is made possible by significant movements of the thumb, finger, and beta-binding domains relative to their orientations in the unliganded polymerase structure. Additionally, the DNA and incoming nucleotide are bound to the active site of Pol IIIalpha nearly identically as they are in their complex with DNA polymerase beta, thereby proving that the eubacterial replicating polymerase, but not the eukaryotic replicating polymerase, is homologous to DNA polymerase beta. Finally, superimposing a recent structure of the clamp bound to DNA on this Pol IIIalpha complex with DNA places a loop of the beta-binding domain into the appropriate clamp cleft and supports a mechanism of polymerase switching.

PubMed: 18691598
DOI: 10.1016/j.jmb.2008.07.058

実験手法

X-RAY DIFFRACTION (4.6 Å)