3DXJ

Crystal structure of thermus thermophilus rna polymerase holoenzyme in complex with the antibiotic myxopyronin

Summary for 3DXJ

• Entry DOI: 10.2210/pdb3dxj/pdb
• Related: 1IW7 1ZYR
• Descriptor: DNA-directed RNA polymerase subunit alpha; CHAIN A, B, K, L, methyl [(1E,5R)-5-{(3S)-3-[(2E,4E)-2,5-dimethylocta-2,4-dienoyl]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hexylidene]carbamate, BACTERIAL RNA POLYMERASE BETA SUBUNIT; CHAIN C, M, ... (11 entities in total)
• Functional Keywords: rna polymerase; rnap; drug complex; inhibitor; corallopyronin; ripostatin; transcription; holoenzyme; crystallography; twinning; hemihedral, dna-directed rna polymerase, nucleotidyltransferase, transcription, transferase, dna-binding, sigma factor, transcription regulation
• Biological source: Thermus thermophilus HB8; More
• Total number of polymer chains: 12
• Total formula weight: 855045.03

Authors

Das, K.,Arnold, E. (deposition date: 2008-07-24, release date: 2008-10-14, Last modification date: 2023-08-30)

• Primary citation: Mukhopadhyay, J.,Das, K.,Ismail, S.,Koppstein, D.,Jang, M.,Hudson, B.,Sarafianos, S.,Tuske, S.,Patel, J.,Jansen, R.,Irschik, H.,Arnold, E.,Ebright, R.H.; The RNA polymerase "switch region" is a target for inhibitors.; Cell(Cambridge,Mass.), 135:295-307, 2008; PubMed: 18957204; DOI: 10.1016/j.cell.2008.09.033

Experimental method

X-RAY DIFFRACTION (3 Å)