3DXJ
Crystal structure of thermus thermophilus rna polymerase holoenzyme in complex with the antibiotic myxopyronin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, G, H (A, B, K, L) | DNA-directed RNA polymerase subunit alpha; CHAIN A, B, K, L | polymer | 315 | 35056.2 | 4 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Thermus thermophilus HB8 | |
| 2 | C, I (C, M) | BACTERIAL RNA POLYMERASE BETA SUBUNIT; CHAIN C, M | polymer | 1119 | 125436.5 | 2 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus HB8 | |
| 3 | D, J (D, N) | BACTERIAL RNA POLYMERASE BETA-PRIME SUBUNIT; CHAIN D, N | polymer | 1524 | 170997.4 | 2 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus HB8 | |
| 4 | E, K (E, O) | BACTERIAL RNA POLYMERASE OMEGA SUBUNIT; CHAIN E, O | polymer | 99 | 11521.2 | 2 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus HB8 | |
| 5 | F, L (F, P) | RNA polymerase pricipal sigma factor (RpoD); CHAIN F, P | polymer | 423 | 48598.0 | 2 | UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Thermus thermophilus HB8 | |
| 6 | M, T (A, D) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 7 | N, S, Y (B, D, N) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 8 | O, P, V (C, M) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
| 9 | Q, R, W, X (D, N) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 10 | U, Z (D, N) | methyl [(1E,5R)-5-{(3S)-3-[(2E,4E)-2,5-dimethylocta-2,4-dienoyl]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hexylidene]carbamate | non-polymer | 417.5 | 2 | Chemie (NE6) | |||
| 11 | AA, BA, CA, DA, EA... (A, B, C, D, F...) | water | water | 18.0 | 30 | Chemie (HOH) |
Sequence modifications
E, O: 1 - 99 (UniProt: Q8RQE7)
| PDB | External Database | Details |
|---|---|---|
| Glu 61 | Val 61 | variant |
| Ile 92 | Leu 92 | variant |
| Gly 95 | Val 95 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 853331.0 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1714.0 | |
| All* | Total formula weight | 855045.0 |
