3DXJ

Crystal structure of thermus thermophilus rna polymerase holoenzyme in complex with the antibiotic myxopyronin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, K, L	DNA-directed RNA polymerase subunit alpha; CHAIN A, B, K, L	polymer	315	35056.2	4	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Thermus thermophilus HB8
2	C, M	BACTERIAL RNA POLYMERASE BETA SUBUNIT; CHAIN C, M	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Thermus thermophilus HB8
3	D, N	BACTERIAL RNA POLYMERASE BETA-PRIME SUBUNIT; CHAIN D, N	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermus thermophilus HB8
4	E, O	BACTERIAL RNA POLYMERASE OMEGA SUBUNIT; CHAIN E, O	polymer	99	11521.2	2	UniProt (Q8RQE7) Pfam (PF01192) In PDB	Thermus thermophilus HB8
5	F, P	RNA polymerase pricipal sigma factor (RpoD); CHAIN F, P	polymer	423	48598.0	2	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Thermus thermophilus HB8
6	A, D	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
7	B, D, N	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	C, M	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
9	D, N	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
10	D, N	methyl [(1E,5R)-5-{(3S)-3-[(2E,4E)-2,5-dimethylocta-2,4-dienoyl]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hexylidene]carbamate	non-polymer		417.5	2	Chemie (NE6)
11		water	water		18.0	30	Chemie (HOH)

Sequence modifications

E, O: 1 - 99 (UniProt: Q8RQE7)

PDB	External Database	Details
Glu 61	Val 61	variant
Ile 92	Leu 92	variant
Gly 95	Val 95	variant

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	853331.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1714.0
All*	Total formula weight	855045.0

*Water molecules are not included.