3DP5

Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II

3DP5 の概要

• エントリーDOI: 10.2210/pdb3dp5/pdb
• 分子名称: Cytochrome c family protein, SULFATE ION, HEME C, ... (4 entities in total)
• 機能のキーワード: c-type cytochrome, fe sad phasing, dissimilatory metal reduction, geobacter sulfurreducens, electron transport
• 由来する生物種: Geobacter sulfurreducens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11066.18

構造登録者

Lukat, P.,Hoffmann, M.,Einsle, O. (登録日: 2008-07-07, 公開日: 2008-09-02, 最終更新日: 2024-10-30)

主引用文献

Lukat, P.,Hoffmann, M.,Einsle, O.
Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II
ACTA CRYSTALLOGR.,SECT.D, 64:919-926, 2008

PubMed Abstract: The putative outer membrane c-type cytochrome OmcF from Geobacter sulfurreducens contains a single haem group and shows homology to soluble cytochromes c(6), a class of electron-transfer proteins that are typically found in cyanobacterial photosynthetic electron-transfer chains. OmcF was overexpressed heterologously in Escherichia coli as an N-terminal Strep-tag II fusion protein and isolated using streptactin-affinity chromatography followed by size-exclusion chromatography. The structure was solved by Fe SAD using data collected to a resolution of 1.86 A on a rotating copper-anode X-ray generator. In the crystal, packing interactions in one dimension were exclusively mediated through the Strep-tag II sequence. The tag and linker regions were in contact with three further monomers of OmcF, leading to a well defined electron-density map for this engineered and secondary-structure-free region of the molecule.

PubMed: 18703839
DOI: 10.1107/S0907444908021306

実験手法

X-RAY DIFFRACTION (1.86 Å)