3DH7
Structure of T. thermophilus IDI-2 in complex with PPi
Summary for 3DH7
| Entry DOI | 10.2210/pdb3dh7/pdb |
| Descriptor | Isopentenyl-diphosphate delta-isomerase, FLAVIN MONONUCLEOTIDE, PYROPHOSPHATE 2-, ... (4 entities in total) |
| Functional Keywords | idi, complex, isomerase, plasmid |
| Biological source | Thermus thermophilus HB8 |
| Cellular location | Cytoplasm (By similarity): Q53W52 |
| Total number of polymer chains | 4 |
| Total formula weight | 146444.71 |
| Authors | de Ruyck, J.,Wouters, J. (deposition date: 2008-06-17, release date: 2008-09-23, Last modification date: 2024-03-20) |
| Primary citation | de Ruyck, J.,Pouyez, J.,Rothman, S.C.,Poulter, D.,Wouters, J. Crystal structure of type 2 isopentenyl diphosphate isomerase from Thermus thermophilus in complex with inorganic pyrophosphate Biochemistry, 47:9051-9053, 2008 Cited by PubMed Abstract: The N-terminal region is stabilized in the crystal structure of Thermus thermophilus type 2 isopentenyl diphosphate isomerase in complex with inorganic pyrophosphate, providing new insights about the active site and the catalytic mechanism of the enzyme. The PP i moiety is located near the conserved residues, H10, R97, H152, Q157, E158, and W219, and the flavin cofactor. The putative active site of isopentenyl diphosphate isomerase 2 provides interactions for stabilizing a carbocationic intermediate similar to those that stabilize the intermediate in the well-established protonation-deprotonation mechanism of isopentenyl diphosphate isomerase 1. PubMed: 18693754DOI: 10.1021/bi801159x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.97 Å) |
Structure validation
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