3DDX
HK97 bacteriophage capsid Expansion Intermediate-II model
Summary for 3DDX
| Entry DOI | 10.2210/pdb3ddx/pdb |
| Related | 1OHG 2FTE 2GP1 |
| Descriptor | Major capsid protein (1 entity in total) |
| Functional Keywords | bacteriophage, hk97, capsid protein, expansion intermediate, virion, icosahedral virus, virus |
| Biological source | Bacteriophage HK97 |
| Total number of polymer chains | 7 |
| Total formula weight | 215632.25 |
| Authors | Lee, K.K.,Gan, L.,Conway, J.F.,Hendrix, R.W.,Steven, A.C.,Johnson, J.E. (deposition date: 2008-06-06, release date: 2008-11-04, Last modification date: 2025-03-05) |
| Primary citation | Lee, K.K.,Gan, L.,Tsuruta, H.,Moyer, C.,Conway, J.F.,Duda, R.L.,Hendrix, R.W.,Steven, A.C.,Johnson, J.E. Virus capsid expansion driven by the capture of mobile surface loops. Structure, 16:1491-1502, 2008 Cited by PubMed Abstract: The capsids of tailed-DNA bacteriophages first assemble as procapsids, which mature by converting into a new form that is strong enough to contain a densely packed viral chromosome. We demonstrate that the intersubunit crosslinking that occurs during maturation of HK97 capsids actually promotes the structural transformation. Small-angle X-ray scattering and crosslinking assays reveal that a shift in the crosslink pattern accompanies conversion of a semimature particle, Expansion Intermediate-I/II, to a more mature state, Balloon. This transition occurs in a switch-like fashion. We find that crosslink formation shifts the global conformational balance to favor the balloon state. A pseudoatomic model of EI-I/II derived from cryo-EM provides insight into the relationship between crosslink formation and conformational switching. PubMed: 18940605DOI: 10.1016/j.str.2008.06.014 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (14 Å) |
Structure validation
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