3CXK

1.7 A Crystal structure of methionine-R-sulfoxide reductase from Burkholderia pseudomallei: crystallization in a microfluidic crystal card.

Summary for 3CXK

• Entry DOI: 10.2210/pdb3cxk/pdb
• Related: 3CEZ
• Descriptor: Methionine-R-sulfoxide reductase, ZINC ION, ACETATE ION, ... (4 entities in total)
• Functional Keywords: structural genomics, burkholderia, msrb, oxidoreductase, microfluidic labcard, psi-2, protein structure initiative, accelerated technologies center for gene to 3d structure, atcg3d, seattle structural genomics center for infectious disease, ssgcid
• Biological source: Burkholderia pseudomallei strain
• Total number of polymer chains: 2
• Total formula weight: 37247.97

Authors

Lovell, S.,Gerdts, C.,Staker, B.,Craigen, D.,Stewart, L.,Accelerated Technologies Center for Gene to 3D Structure (ATCG3D),Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2008-04-24, release date: 2008-05-27, Last modification date: 2023-08-30)

Primary citation

Gerdts, C.J.,Elliott, M.,Lovell, S.,Mixon, M.B.,Napuli, A.J.,Staker, B.L.,Nollert, P.,Stewart, L.
The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).
Acta Crystallogr.,Sect.D, 64:1116-1122, 2008

PubMed Abstract: The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, approximately 10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comprehensive ;hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.

PubMed: 19020349
DOI: 10.1107/S0907444908028060

Experimental method

X-RAY DIFFRACTION (1.7 Å)