3CW1

Crystal Structure of Human Spliceosomal U1 snRNP

3CW1 の概要

• エントリーDOI: 10.2210/pdb3cw1/pdb
• 分子名称: U1 snRNA, U1 small nuclear ribonucleoprotein C, ZINC ION, ... (11 entities in total)
• 機能のキーワード: pre-mrna splicing, spliceosome, rna-binding domain, sm fold, zinc finger, rna recognition motif, 5' splice site, splicing
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, cytosol : 3CW1 P62318 P14678 P62314 P62316 P62306 P62304; Nucleus: P62308 P08621
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 666156.96

構造登録者

Pomeranz Krummel, D.A.,Oubridge, C.,Leung, A.K.,Li, J.,Nagai, K. (登録日: 2008-04-21, 公開日: 2009-03-24, 最終更新日: 2024-02-21)

主引用文献

Pomeranz Krummel, D.A.,Oubridge, C.,Leung, A.K.,Li, J.,Nagai, K.
Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution.
Nature, 458:475-480, 2009

PubMed Abstract: Human spliceosomal U1 small nuclear ribonucleoprotein particles (snRNPs), which consist of U1 small nuclear RNA and ten proteins, recognize the 5' splice site within precursor messenger RNAs and initiate the assembly of the spliceosome for intron excision. An electron density map of the functional core of U1 snRNP at 5.5 A resolution has enabled us to build the RNA and, in conjunction with site-specific labelling of individual proteins, to place the seven Sm proteins, U1-C and U1-70K into the map. Here we present the detailed structure of a spliceosomal snRNP, revealing a hierarchical network of intricate interactions between subunits. A striking feature is the amino (N)-terminal polypeptide of U1-70K, which extends over a distance of 180 A from its RNA binding domain, wraps around the core domain consisting of the seven Sm proteins and finally contacts U1-C, which is crucial for 5'-splice-site recognition. The structure of U1 snRNP provides insights into U1 snRNP assembly and suggests a possible mechanism of 5'-splice-site recognition.

PubMed: 19325628
DOI: 10.1038/nature07851

実験手法

X-RAY DIFFRACTION (5.493 Å)