3CF4

Structure of the CODH component of the M. barkeri ACDS complex

3CF4 の概要

• エントリーDOI: 10.2210/pdb3cf4/pdb
• 分子名称: Acetyl-CoA decarboxylase/synthase alpha subunit, Acetyl-CoA decarboxylase/synthase epsilon subunit, IRON/SULFUR CLUSTER, ... (10 entities in total)
• 機能のキーワード: methanomicrobia, iron-nikel-sulfur, 4fe-ni-4s, oxidoreductase
• 由来する生物種: Methanosarcina barkeri; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110019.32

構造登録者

Gong, W.,Hao, B.,Wei, Z.,Ferguson Jr., D.J.,Tallant, T.,Krzycki, J.A.,Chan, M.K. (登録日: 2008-03-01, 公開日: 2008-07-22, 最終更新日: 2024-02-21)

主引用文献

Gong, W.,Hao, B.,Wei, Z.,Ferguson Jr., D.J.,Tallant, T.,Krzycki, J.A.,Chan, M.K.
Structure of the alpha2 epsilon2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarboxylase/synthase complex
Proc.Natl.Acad.Sci.USA, 105:9558-9563, 2008

PubMed Abstract: Ni-dependent carbon monoxide dehydrogenases (Ni-CODHs) are a diverse family of enzymes that catalyze reversible CO:CO(2) oxidoreductase activity in acetogens, methanogens, and some CO-using bacteria. Crystallography of Ni-CODHs from CO-using bacteria and acetogens has revealed the overall fold of the Ni-CODH core and has suggested structures for the C cluster that mediates CO:CO(2) interconversion. Despite these advances, the mechanism of CO oxidation has remained elusive. Herein, we report the structure of a distinct class of Ni-CODH from methanogenic archaea: the alpha(2)epsilon(2) component from the alpha(8)beta(8)gamma(8)delta(8)epsilon(8) CODH/acetyl-CoA decarbonylase/synthase complex, an enzyme responsible for the majority of biogenic methane production on Earth. The structure of this Ni-CODH component provides support for a hitherto unobserved state in which both CO and H(2)O/OH(-) bind to the Ni and the exogenous FCII iron of the C cluster, respectively, and offers insight into the structures and functional roles of the epsilon-subunit and FeS domain not present in nonmethanogenic Ni-CODHs.

PubMed: 18621675
DOI: 10.1073/pnas.0800415105

実験手法

X-RAY DIFFRACTION (2 Å)