3CBB

Crystal Structure of Hepatocyte Nuclear Factor 4alpha in complex with DNA: Diabetes Gene Product

3CBB の概要

• エントリーDOI: 10.2210/pdb3cbb/pdb
• 分子名称: Hepatocyte Nuclear Factor 4-alpha promoter element DNA, Hepatocyte Nuclear Factor 4-alpha, DNA binding domain, ZINC ION, ... (5 entities in total)
• 機能のキーワード: zinc finger, protein-dna complex, diabetes, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P41235
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31260.15

構造登録者

Lu, P.,Rha, G.B.,Melikishvili, M.,Adkins, B.C.,Fried, M.G.,Chi, Y.I. (登録日: 2008-02-21, 公開日: 2008-10-07, 最終更新日: 2024-02-21)

主引用文献

Lu, P.,Rha, G.B.,Melikishvili, M.,Wu, G.,Adkins, B.C.,Fried, M.G.,Chi, Y.I.
Structural basis of natural promoter recognition by a unique nuclear receptor, HNF4alpha. Diabetes gene product.
J.Biol.Chem., 283:33685-33697, 2008

PubMed Abstract: HNF4alpha (hepatocyte nuclear factor 4alpha) plays an essential role in the development and function of vertebrate organs, including hepatocytes and pancreatic beta-cells by regulating expression of multiple genes involved in organ development, nutrient transport, and diverse metabolic pathways. As such, HNF4alpha is a culprit gene product for a monogenic and dominantly inherited form of diabetes, known as maturity onset diabetes of the young (MODY). As a unique member of the nuclear receptor superfamily, HNF4alpha recognizes target genes containing two hexanucleotide direct repeat DNA-response elements separated by one base pair (DR1) by exclusively forming a cooperative homodimer. We describe here the 2.0 angstroms crystal structure of human HNF4alpha DNA binding domain in complex with a high affinity promoter element of another MODY gene, HNF1alpha, which reveals the molecular basis of unique target gene selection/recognition, DNA binding cooperativity, and dysfunction caused by diabetes-causing mutations. The predicted effects of MODY mutations have been tested by a set of biochemical and functional studies, which show that, in contrast to other MODY gene products, the subtle disruption of HNF4alpha molecular function can cause significant effects in afflicted MODY patients.

PubMed: 18829458
DOI: 10.1074/jbc.M806213200

実験手法

X-RAY DIFFRACTION (2 Å)