3C98

Revised structure of the munc18a-syntaxin1 complex

「1DN1」から置き換えられました

3C98 の概要

• エントリーDOI: 10.2210/pdb3c98/pdb
• 関連するPDBエントリー: 1DN1
• 分子名称: Syntaxin-binding protein 1, Syntaxin-1A (3 entities in total)
• 機能のキーワード: protein complex, alternative splicing, cytoplasm, membrane, phosphoprotein, protein transport, transport, coiled coil, neurotransmitter transport, transmembrane, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasm: P61765; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101522.69

構造登録者

Hattendorf, D.A.,Misura, K.M.,Burkhardt, P.,Scheller, R.H.,Fasshauer, D.,Weis, W.I. (登録日: 2008-02-15, 公開日: 2008-03-25, 最終更新日: 2024-03-13)

主引用文献

Burkhardt, P.,Hattendorf, D.A.,Weis, W.I.,Fasshauer, D.
Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide
Embo J., 27:923-933, 2008

PubMed Abstract: Sec1/Munc18-like (SM) proteins functionally interact with SNARE proteins in vesicular fusion. Despite their high sequence conservation, structurally disparate binding modes for SM proteins with syntaxins have been observed. Several SM proteins appear to bind only to a short peptide present at the N terminus of syntaxin, designated the N-peptide, while Munc18a binds to a 'closed' conformation formed by the remaining portion of syntaxin 1a. Here, we show that the syntaxin 16 N-peptide binds to the SM protein Vps45, but the remainder of syntaxin 16 strongly enhances the affinity of the interaction. Likewise, the N-peptide of syntaxin 1a serves as a second binding site in the Munc18a/syntaxin 1a complex. When the syntaxin 1a N-peptide is bound to Munc18a, SNARE complex formation is blocked. Removal of the N-peptide enables binding of syntaxin 1a to its partner SNARE SNAP-25, while still bound to Munc18a. This suggests that Munc18a controls the accessibility of syntaxin 1a to its partners, a role that might be common to all SM proteins.

PubMed: 18337752
DOI: 10.1038/emboj.2008.37

実験手法

X-RAY DIFFRACTION (2.601 Å)