3C8E

Crystal Structure Analysis of yghU from E. Coli

3C8E の概要

• エントリーDOI: 10.2210/pdb3c8e/pdb
• 分子名称: yghU, glutathione S-transferase homologue, GLUTATHIONE (3 entities in total)
• 機能のキーワード: glutathione transferase homologue, yghu, e. coli, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66092.39

構造登録者

Harp, J.,Ladner, J.E.,Schaab, M.R.,Stourman, N.V.,Armstrong, R.N. (登録日: 2008-02-11, 公開日: 2009-02-24, 最終更新日: 2024-02-21)

主引用文献

Stourman, N.V.,Branch, M.C.,Schaab, M.R.,Harp, J.M.,Ladner, J.E.,Armstrong, R.N.
Structure and Function of YghU, a Nu-Class Glutathione Transferase Related to YfcG from Escherichia coli.
Biochemistry, 50:1274-1281, 2011

PubMed Abstract: The crystal structure (1.50 Å resolution) and biochemical properties of the GSH transferase homologue, YghU, from Escherichia coli reveal that the protein is unusual in that it binds two molecules of GSH in each active site. The crystallographic observation is consistent with biphasic equilibrium binding data that indicate one tight (K(d1) = 0.07 ± 0.03 mM) and one weak (K(d2) = 1.3 ± 0.2 mM) binding site for GSH. YghU exhibits little or no GSH transferase activity with most typical electrophilic substrates but does possess a modest catalytic activity toward several organic hydroperoxides. Most notably, the enzyme also exhibits disulfide-bond reductase activity toward 2-hydroxyethyl disulfide [k(cat) = 74 ± 6 s(-1), and k(cat)/K(M)(GSH) = (6.6 ± 1.3) × 10(4) M(-1) s(-1)] that is comparable to that previously determined for YfcG. A superposition of the structures of the YghU·2GSH and YfcG·GSSG complexes reveals a remarkable structural similarity of the active sites and the 2GSH and GSSG molecules in each. We conclude that the two structures represent reduced and oxidized forms of GSH-dependent disulfide-bond oxidoreductases that are distantly related to glutaredoxin 2. The structures and properties of YghU and YfcG indicate that they are members of the same, but previously unidentified, subfamily of GSH transferase homologues, which we suggest be called the nu-class GSH transferases.

PubMed: 21222452
DOI: 10.1021/bi101861a

実験手法

X-RAY DIFFRACTION (1.5 Å)