3BW2

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

Summary for 3BW2

• Entry DOI: 10.2210/pdb3bw2/pdb
• Related: 3BW3 3BW4
• Descriptor: 2-nitropropane dioxygenase, FLAVIN MONONUCLEOTIDE, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• Functional Keywords: tim barrel, oxidoreductase, dioxygenase
• Biological source: Streptomyces ansochromogenes
• Total number of polymer chains: 1
• Total formula weight: 39118.88

Authors

Li, Y.H.,Gao, Z.Q.,Hou, H.F. (deposition date: 2008-01-08, release date: 2009-01-13, Last modification date: 2024-03-13)

Primary citation

Li, Y.H.,Gao, Z.Q.,Hou, H.F.,Li, L.,Zhang, J.H.,Yang, H.H.,Dong, Y.H.,Tan, H.R.
Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes
Biochem.Biophys.Res.Commun., 405:344-348, 2011

PubMed Abstract: Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His179, which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure.

PubMed: 21147069
DOI: 10.1016/j.bbrc.2010.12.050

Experimental method

X-RAY DIFFRACTION (2.1 Å)