3BUY

MHC-I in complex with peptide

Summary for 3BUY

• Entry DOI: 10.2210/pdb3buy/pdb
• Descriptor: H-2 class I histocompatibility antigen, D-B alpha chain, Beta-2-microglobulin, epitope of PB1-F2, ... (4 entities in total)
• Functional Keywords: mhc-i, glycoprotein, immune response, membrane, mhc i, transmembrane, immunoglobulin domain, polymorphism, secreted, apoptosis, cytoplasm, host-virus interaction, inner membrane, mitochondrion, nucleus, immune system
• Biological source: Mus musculus (house mouse); More
• Cellular location: Membrane; Single-pass type I membrane protein: P01899; Secreted: P01887; Host mitochondrion inner membrane: P0C0U1
• Total number of polymer chains: 3
• Total formula weight: 44760.27

Authors

Rossjohn, J.,La Gruta, N.L.,Purcell, A.W.,Turner, S.J.,Dunstone, M.A. (deposition date: 2008-01-03, release date: 2008-03-25, Last modification date: 2024-11-13)

Primary citation

La Gruta, N.L.,Thomas, P.G.,Webb, A.I.,Dunstone, M.A.,Cukalac, T.,Doherty, P.C.,Purcell, A.W.,Rossjohn, J.,Turner, S.J.
Epitope-specific TCRbeta repertoire diversity imparts no functional advantage on the CD8+ T cell response to cognate viral peptides
Proc.Natl.Acad.Sci.Usa, 105:2034-2039, 2008

PubMed Abstract: TCR repertoire diversity has been convincingly shown to facilitate responsiveness of CD8+ T cell populations to mutant virus peptides, thereby safeguarding against viral escape. However, the impact of repertoire diversity on the functionality of the CD8+ T cell response to cognate peptide-MHC class I complex (pMHC) recognition remains unclear. Here, we have compared TCRbeta chain repertoires of three influenza A epitope-specific CD8+ T cell responses in C57BL/6 (B6) mice: D(b)NP(366-374), D(b)PA(224-233), and a recently described epitope derived from the +1 reading frame of the influenza viral polymerase B subunit (residues 62-70) (D(b)PB1-F2(62)). Corresponding to the relative antigenicity of the respective pMHCs, and irrespective of the location of prominent residues, the D(b)PA(224)- and D(b)PB1-F2(62)-specific repertoires were similarly diverse, whereas the D(b)NP(366) population was substantially narrower. Importantly, parallel analysis of response magnitude, cytotoxicity, TCR avidity, and cytokine production for the three epitope-specific responses revealed no obvious functional advantage conferred by increased T cell repertoire diversity. Thus, whereas a diverse repertoire may be important for recognition of epitope variants, its effect on the response to cognate pMHC recognition appears minimal.

PubMed: 18238896
DOI: 10.1073/pnas.0711682102

Experimental method

X-RAY DIFFRACTION (2.6 Å)