3BQ0

Pre-insertion binary complex of Dbh DNA polymerase

Summary for 3BQ0

• Entry DOI: 10.2210/pdb3bq0/pdb
• Related: 3BQ1 3BQ2
• Descriptor: DNA polymerase IV, DNA (5'-D(*DGP*DAP*DAP*DGP*DCP*DCP*DGP*DGP*DCP*DG)-3'), DNA (5'-D(*DT*DTP*DCP*DCP*DGP*DCP*DCP*DCP*DGP*DGP*DCP*DTP*DTP*DCP*DC)-3'), ... (5 entities in total)
• Functional Keywords: dna polymerase, y-family, lesion bypass, single-base deletion, -1 frameshift, dna damage, dna repair, dna replication, dna-binding, dna-directed dna polymerase, magnesium, metal-binding, mutator protein, nucleotidyltransferase, transferase, transferase/dna, transferase-dna complex
• Biological source: Sulfolobus acidocaldarius; More
• Cellular location: Cytoplasm : Q4JB80
• Total number of polymer chains: 3
• Total formula weight: 47653.83

Authors

Pata, J.D.,Wilson, R.C. (deposition date: 2007-12-19, release date: 2008-04-08, Last modification date: 2023-08-30)

Primary citation

Wilson, R.C.,Pata, J.D.
Structural insights into the generation of single-base deletions by the Y family DNA polymerase dbh.
Mol.Cell, 29:767-779, 2008

PubMed Abstract: Dbh is a Y family translesion DNA polymerase that accurately bypasses some damaged forms of deoxyguanosine, but also generates single-base deletion errors at frequencies of up to 50%, in specific hot spot sequences. We describe preinsertion binary, insertion ternary, and postinsertion binary crystal structures of Dbh synthesizing DNA after making a single-base deletion. The skipped template base adopts an extrahelical conformation stabilized by interactions with the C-terminal domain of the enzyme. DNA translocation and positioning of the next templating base at the active site, with space opposite to accommodate incoming nucleotide, occur independently of nucleotide binding, incorporation, and pyrophosphate release. We also show that Dbh creates single-base deletions more rapidly when the skipped base is located two or three bases upstream of the nascent base pair than when it is directly adjacent to the templating base, indicating that Dbh predominantly creates single-base deletions by template slippage rather than by dNTP-stabilized misalignment.

PubMed: 18374650
DOI: 10.1016/j.molcel.2008.01.014

Experimental method

X-RAY DIFFRACTION (2.6 Å)