3BOD

Structure of mouse beta-neurexin 1

Summary for 3BOD

• Entry DOI: 10.2210/pdb3bod/pdb
• Descriptor: Neurexin-1-alpha, CALCIUM ION (3 entities in total)
• Functional Keywords: neurexin1d, lns6, alternative splicing, calcium, cell adhesion, egf-like domain, glycoprotein, membrane, metal-binding, transmembrane
• Biological source: Mus musculus (house mouse); More
• Cellular location: Cell membrane ; Single-pass type I membrane protein : Q9CS84
• Total number of polymer chains: 1
• Total formula weight: 19351.79

Authors

Koehnke, J.,Jin, X.,Shapiro, L. (deposition date: 2007-12-17, release date: 2008-03-25, Last modification date: 2024-02-21)

Primary citation

Koehnke, J.,Jin, X.,Trbovic, N.,Katsamba, P.S.,Brasch, J.,Ahlsen, G.,Scheiffele, P.,Honig, B.,Palmer, A.G.,Shapiro, L.
Crystal Structures of beta-Neurexin 1 and beta-Neurexin 2 Ectodomains and Dynamics of Splice Insertion Sequence 4.
Structure, 16:410-421, 2008

PubMed Abstract: Presynaptic neurexins (NRXs) bind to postsynaptic neuroligins (NLs) to form Ca(2+)-dependent complexes that bridge neural synapses. beta-NRXs bind NLs through their LNS domains, which contain a single site of alternative splicing (splice site 4) giving rise to two isoforms: +4 and Delta. We present crystal structures of the Delta isoforms of the LNS domains from beta-NRX1 and beta-NRX2, crystallized in the presence of Ca(2+) ions. The Ca(2+)-binding site is disordered in the beta-NRX2 structure, but the 1.7 A beta-NRX1 structure reveals a single Ca(2+) ion, approximately 12 A from the splice insertion site, with one coordinating ligand donated by a glutamic acid from an adjacent beta-NRX1 molecule. NMR studies of beta-NRX1+4 show that the insertion sequence is unstructured, and remains at least partially disordered in complex with NL. These results raise the possibility that beta-NRX insertion sequence 4 may function in roles independent of neuroligin binding.

PubMed: 18334216
DOI: 10.1016/j.str.2007.12.024

Experimental method

X-RAY DIFFRACTION (1.7 Å)