3BNR

Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the presence of nonspecifically bound paromomycin (A1555G mutant, Br-derivative)

Summary for 3BNR

• Entry DOI: 10.2210/pdb3bnr/pdb
• Related: 3BNL 3BNN 3BNO 3BNP 3BNQ 3BNS 3BNT
• Descriptor: A site of human mitochondrial ribosome, chain one, A site of human mitochondrial ribosome, chain two, A site of human mitochondrial ribosome, chain three, ... (6 entities in total)
• Functional Keywords: ribosome, decoding site, rna
• Total number of polymer chains: 4
• Total formula weight: 29352.45

Authors

Kondo, J.,Westhof, E. (deposition date: 2007-12-14, release date: 2008-06-24, Last modification date: 2024-02-21)

Primary citation

Kondo, J.,Westhof, E.
The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates
Nucleic Acids Res., 36:2654-2666, 2008

PubMed Abstract: The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation.

PubMed: 18346970
DOI: 10.1093/nar/gkn112

Experimental method

X-RAY DIFFRACTION (2.1 Å)