3BNR
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the presence of nonspecifically bound paromomycin (A1555G mutant, Br-derivative)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.189, 77.380, 56.322 |
Unit cell angles | 90.00, 115.12, 90.00 |
Refinement procedure
Resolution | 50.995 - 2.100 |
Rwork | 0.215 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.900 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.995 | 50.990 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.116 | 0.089 | 0.378 |
Total number of observations | 3289 | 16679 | |
Number of reflections | 16388 | ||
<I/σ(I)> | 2.7 | 6 | 1.5 |
Completeness [%] | 99.8 | 96 | 100 |
Redundancy | 6.9 | 6.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Sodium cacodylate | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | KCl |