3BIM

Crystal structure of the BCL6 BTB domain dimer in complex with the BCOR BBD corepressor peptide

3BIM の概要

• エントリーDOI: 10.2210/pdb3bim/pdb
• 分子名称: B-cell lymphoma 6 protein, BCL-6 corepressor (3 entities in total)
• 機能のキーワード: protein-peptide compex, activator, chromosomal rearrangement, dna-binding, metal-binding, nucleus, phosphoprotein, polymorphism, proto-oncogene, repressor, transcription, transcription regulation, zinc, zinc-finger, alternative splicing, ank repeat, chromatin regulator, disease mutation, transcription repressor
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P41182 Q6W2J9
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 131903.61

構造登録者

Prive, G.G.,Ghetu, A.F. (登録日: 2007-11-30, 公開日: 2008-01-08, 最終更新日: 2023-08-30)

主引用文献

Ghetu, A.F.,Corcoran, C.M.,Cerchietti, L.,Bardwell, V.J.,Melnick, A.,Prive, G.G.
Structure of a BCOR corepressor peptide in complex with the BCL6 BTB domain dimer.
Mol.Cell, 29:384-391, 2008

PubMed Abstract: The transcriptional corepressors BCOR, SMRT, and NCoR are known to bind competitively to the BCL6 BTB domain despite the fact that BCOR has no detectable sequence similarity to the other two corepressors. We have identified a 17 residue motif from BCOR that binds directly to the BCL6 BTB domain and determined the crystal structure of the complex to a resolution of 2.6 A. Remarkably, the BCOR BCL6 binding domain (BCOR(BBD)) peptide binds in the same BCL6 binding site as the SMRT(BBD) peptide despite the lack of any significant sequence similarity between the two peptides. Mutations of critical BCOR(BBD) residues cause the disruption of the BCL6 corepression activities of BCOR, and a BCOR(BBD) peptide blocks BCL6-mediated transcriptional repression and kills lymphoma cells.

PubMed: 18280243
DOI: 10.1016/j.molcel.2007.12.026

実験手法

X-RAY DIFFRACTION (2.6 Å)