3B71

CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

3B71 の概要

• エントリーDOI: 10.2210/pdb3b71/pdb
• 関連するPDBエントリー: 1K04 1K05 1OW6 1OW7 1OW8
• 分子名称: Focal adhesion kinase 1, T-cell surface glycoprotein CD4 (2 entities in total)
• 機能のキーワード: four-helix bundle, protein-protein complex, atp-binding, cell junction, kinase, nucleotide-binding, phosphorylation, transferase, tyrosine-protein kinase, glycoprotein, host-virus interaction, immune response, immunoglobulin domain, lipoprotein, membrane, palmitate, transmembrane, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell junction, focal adhesion: Q05397; Cell membrane; Single-pass type I membrane protein: P01730
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 62184.00

構造登録者

Garron, M.-L.,Arold, S.T. (登録日: 2007-10-30, 公開日: 2008-01-01, 最終更新日: 2023-08-30)

主引用文献

Garron, M.L.,Arthos, J.,Guichou, J.F.,McNally, J.,Cicala, C.,Arold, S.T.
Structural basis for the interaction between focal adhesion kinase and CD4.
J.Mol.Biol., 375:1320-1328, 2008

PubMed Abstract: Focal adhesion kinase (FAK) and CD4 fulfil vital functions in cellular signal transduction: FAK is a central component in integrin signalling, whereas CD4 plays essential roles in the immune defence. In T lymphocytes, FAK and CD4 localise to the same signalling complexes after stimulation by either the human immunodeficiency virus (HIV) gp120 glycoprotein or an antigen, suggesting the concerted action of FAK and CD4 in these cells. Using crystallography and microcalorimetry, we here show that the focal adhesion targeting (FAT) domain of FAK binds specifically to the CD4 endocytosis motif in vitro. This FAT-CD4 complex is structurally and thermodynamically similar to the one FAT forms with paxillin LD motifs. The CD4 binding site on FAT presents the same features as the established CD4 binding site on the HIV-1 Nef protein. The binding of FAT to CD4 is incompatible with the binding of Lck to CD4. We further show that HIV-1 gp120 triggers the association of CD4 with FAK in T cells, under conditions that are known to dissociate Lck from CD4. Our results suggest that the FAK-CD4 complex represents an alternative route for eliciting T-cell-specific signals and that it links gp120 engagement to distinctive T-cell signalling during HIV infection. In infected cells, HIV-1 Nef may displace FAK from CD4 to protect the cells from apoptosis.

PubMed: 18078954
DOI: 10.1016/j.jmb.2007.11.040

実験手法

X-RAY DIFFRACTION (2.82 Å)