3ATZ

Crystal structure of TcOYE with pHBA

Summary for 3ATZ

• Entry DOI: 10.2210/pdb3atz/pdb
• Related: 3ATY
• Descriptor: Prostaglandin F2a synthase, FLAVIN MONONUCLEOTIDE, P-HYDROXYBENZALDEHYDE, ... (4 entities in total)
• Functional Keywords: alpha/beta barrel, flavin mononucleotide, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• Biological source: Trypanosoma cruzi
• Total number of polymer chains: 4
• Total formula weight: 171565.20

Authors

Okamoto, N.,Yamaguchi, K.,Mizohata, E.,Tokuoka, K.,Uchiyama, N.,Sugiyama, S.,Matsumura, H.,Inaka, K.,Urade, Y.,Inoue, T. (deposition date: 2011-01-26, release date: 2012-02-01, Last modification date: 2024-03-13)

Primary citation

Okamoto, N.,Yamaguchi, K.,Mizohata, E.,Tokuoka, K.,Uchiyama, N.,Sugiyama, S.,Matsumura, H.,Inaka, K.,Urade, Y.,Inoue, T.
Structural insight into the stereoselective production of PGF2(alpha) by Old Yellow Enzyme from Trypanosoma cruzi
J.Biochem., 150:563-568, 2011

PubMed Abstract: Old yellow enzyme (OYE) is an NADPH oxidoreductase capable of reducing a variety of compounds. It contains flavin mononucleotide (FMN) as a prosthetic group. A ternary complex structure of OYE from Trypanosoma cruzi (TcOYE) with FMN and one of the substrates, p-hydroxybenzaldehyde, shows a striking movement around the active site upon binding of the substrate. From a structural comparison of other OYE complexed with 12-oxophytodienoate, we have constructed a complex structure with another substrate, prostaglandin H(2) (PGH(2)), to provide a proposed stereoselective reaction mechanism for the reduction of PGH(2) to prostaglandin F(2α) by TcOYE.

PubMed: 21840922
DOI: 10.1093/jb/mvr096

Experimental method

X-RAY DIFFRACTION (2.04 Å)