3AQ3
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b
Summary for 3AQ3
| Entry DOI | 10.2210/pdb3aq3/pdb |
| Related | 3AQ2 3AQ4 |
| Descriptor | 6b protein (2 entities in total) |
| Functional Keywords | agrobacterium 6b, toxin, adp-ribosylation, mirna machineries |
| Biological source | Agrobacterium tumefaciens |
| Total number of polymer chains | 3 |
| Total formula weight | 73603.93 |
| Authors | Wang, M.M.,Yuan, Y.A. (deposition date: 2010-10-25, release date: 2011-01-26, Last modification date: 2024-10-30) |
| Primary citation | Wang, M.M.,Soyano, T.,Machida, S.,Yang, J.Y.,Jung, C.,Chua, N.H.,Yuan, Y.A. Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b Genes Dev., 25:64-76, 2011 Cited by PubMed Abstract: The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å, Agrobacterium vitis AB6b at 1.65 Å, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 Å. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity. PubMed: 21156810DOI: 10.1101/gad.1985511 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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