3AN1

Crystal structure of rat D428A mutant, urate bound form

Summary for 3AN1

Entry DOI	10.2210/pdb3an1/pdb
Related	3AM9 3AMZ
Descriptor	Xanthine dehydrogenase/oxidase, FE2/S2 (INORGANIC) CLUSTER, CALCIUM ION, ... (8 entities in total)
Functional Keywords	product bound form, oxidoreductase, urate binding
Biological source	Rattus norvegicus (rat)
Cellular location	Peroxisome: P22985
Total number of polymer chains	2
Total formula weight	295817.82
Authors	Okamoto, K.,Kawaguchi, Y.,Eger, B.T.,Pai, E.F.,Nishino, T. (deposition date: 2010-08-27, release date: 2010-12-01, Last modification date: 2023-11-01)
Primary citation	Okamoto, K.,Kawaguchi, Y.,Eger, B.T.,Pai, E.F.,Nishino, T. Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate J.Am.Chem.Soc., 132:17080-17083, 2010 Cited by PubMed Abstract: Two contradictory models have been proposed for the binding mode of the substrate xanthine to and its activation mechanism by xanthine oxidoreductase. In an effort to distinguish between the two models, we determined the crystal structures of the urate complexes of the demolybdo-form of the D428A mutant of rat xanthine oxidoreductase at 1.7 Å and of the reduced bovine milk enzyme at 2.1 Å, the latter representing a reaction intermediate. The results clearly indicate the catalytically relevant binding mode of the substrate xanthine. PubMed: 21077683 DOI: 10.1021/ja1077574 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.73 Å)

Structure validation