3AN1

Crystal structure of rat D428A mutant, urate bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	98.536, 138.242, 222.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.350 - 1.730
R-factor	0.16099
Rwork	0.160
R-free	0.19754
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3am9
RMSD bond length	0.028
RMSD bond angle	2.250
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.400
High resolution limit [Å]	1.730
Number of reflections	300756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	295	9-11% polyethylene glycol 8000, 0.6M Li2SO4, 5mM DTT, 1mM sodium salicylate, 0.4mM EDTA, 15% glycerol, 40mM HEPES (pH 6.20), VAPOR DIFFUSION, HANGING DROP, temperature 295K