3AN1

Crystal structure of rat D428A mutant, urate bound form

3AN1 の概要

• エントリーDOI: 10.2210/pdb3an1/pdb
• 関連するPDBエントリー: 3AM9 3AMZ
• 分子名称: Xanthine dehydrogenase/oxidase, FE2/S2 (INORGANIC) CLUSTER, CALCIUM ION, ... (8 entities in total)
• 機能のキーワード: product bound form, oxidoreductase, urate binding
• 由来する生物種: Rattus norvegicus (rat)
• 細胞内の位置: Peroxisome: P22985
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 295817.82

構造登録者

Okamoto, K.,Kawaguchi, Y.,Eger, B.T.,Pai, E.F.,Nishino, T. (登録日: 2010-08-27, 公開日: 2010-12-01, 最終更新日: 2023-11-01)

主引用文献

Okamoto, K.,Kawaguchi, Y.,Eger, B.T.,Pai, E.F.,Nishino, T.
Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate
J.Am.Chem.Soc., 132:17080-17083, 2010

PubMed Abstract: Two contradictory models have been proposed for the binding mode of the substrate xanthine to and its activation mechanism by xanthine oxidoreductase. In an effort to distinguish between the two models, we determined the crystal structures of the urate complexes of the demolybdo-form of the D428A mutant of rat xanthine oxidoreductase at 1.7 Å and of the reduced bovine milk enzyme at 2.1 Å, the latter representing a reaction intermediate. The results clearly indicate the catalytically relevant binding mode of the substrate xanthine.

PubMed: 21077683
DOI: 10.1021/ja1077574

実験手法

X-RAY DIFFRACTION (1.73 Å)