3ALA

Crystal structure of vascular adhesion protein-1 in space group C2

3ALA の概要

• エントリーDOI: 10.2210/pdb3ala/pdb
• 関連するPDBエントリー: 1pu4 1us1 2c10 2c11
• 分子名称: Membrane primary amine oxidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: membrane primary amine oxidase, vascular adhesion protein-1, vap-1, semicarbazide-sensitive amine oxidase, ssao, copper containing amine oxidase, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type II membrane protein: Q16853
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 595576.36

構造登録者

Ernberg, K.E.,McGrath, A.P.,Guss, J.M. (登録日: 2010-07-29, 公開日: 2010-12-15, 最終更新日: 2023-11-01)

主引用文献

Ernberg, K.,McGrath, A.P.,Peat, T.S.,Adams, T.E.,Xiao, X.,Pham, T.,Newman, J.,McDonald, I.A.,Collyer, C.A.,Guss, J.M.
A new crystal form of human vascular adhesion protein 1
Acta Crystallogr.,Sect.F, 66:1572-1578, 2010

PubMed Abstract: Human vascular adhesion protein 1 (VAP-1) is involved in lymphocyte-endothelial cell adhesion and has been implicated in many human inflammatory diseases. VAP-1 is a member of the copper amine oxidase family of enzymes with a trihydroxyphenylalanine quinone (TPQ) cofactor. Previously characterized crystals of VAP-1 suffered from anisotropy and contained disordered regions; in addition, one form was consistently twinned. In an effort to grow crystals that diffracted to higher resolution for inhibitor-binding studies, a construct with an N-terminal deletion was made and expressed in the Chinese hamster ovary (CHO) glycosylation mutant cell line Lec8. Screening produced crystals that displayed some anisotropy and contained seven molecules per asymmetric unit. These crystals belonged to space group C2, with unit-cell parameters a=394.5, b=115.8, c=179.3 Å, β=112.3°. The structure was refined to a resolution of 2.9 Å, with Rcryst and Rfree values of 0.250 and 0.286, respectively.

PubMed: 21139198
DOI: 10.1107/S1744309110041515

実験手法

X-RAY DIFFRACTION (2.9 Å)