3AJB

Crystal Structure of human Pex3p in complex with N-terminal Pex19p peptide

Summary for 3AJB

• Entry DOI: 10.2210/pdb3ajb/pdb
• Descriptor: Peroxisomal biogenesis factor 3, Peroxisomal biogenesis factor 19 (3 entities in total)
• Functional Keywords: all alpha, protein-protein complex, protein transport
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : P40855
• Total number of polymer chains: 2
• Total formula weight: 41810.62

Authors

Sato, Y.,Shibata, H.,Nakatsu, T.,Kato, H. (deposition date: 2010-05-27, release date: 2010-12-22, Last modification date: 2024-03-13)

Primary citation

Sato, Y.,Shibata, H.,Nakatsu, T.,Nakano, H.,Kashiwayama, Y.,Imanaka, T.,Kato, H.
Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p
Embo J., 29:4083-4093, 2010

PubMed Abstract: Peroxisomes require peroxin (Pex) proteins for their biogenesis. The interaction between Pex3p, which resides on the peroxisomal membrane, and Pex19p, which resides in the cytosol, is crucial for peroxisome formation and the post-translational targeting of peroxisomal membrane proteins (PMPs). It is not known how Pex3p promotes the specific interaction with Pex19p for the purpose of PMP translocation. Here, we present the three-dimensional structure of the complex between a cytosolic domain of Pex3p and the binding-region peptide of Pex19p. The overall shape of Pex3p is a prolate spheroid with a novel fold, the 'twisted six-helix bundle.' The Pex19p-binding site is at an apex of the Pex3p spheroid. A 16-residue region of the Pex19p peptide forms an α-helix and makes a contact with Pex3p; this helix is disordered in the unbound state. The Pex19p peptide contains a characteristic motif, consisting of the leucine triad (Leu18, Leu21, Leu22), and Phe29, which are critical for the Pex3p binding and peroxisome biogenesis.

PubMed: 21102411
DOI: 10.1038/emboj.2010.293

Experimental method

X-RAY DIFFRACTION (2.5 Å)