3AD8

Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate

3AD8 の概要

• エントリーDOI: 10.2210/pdb3ad8/pdb
• 関連するPDBエントリー: 1VRQ 1X31 3AD7 3AD9 3ADA
• 分子名称: SARCOSINE OXIDASE ALPHA SUBUNIT, ZINC ION, SARCOSINE OXIDASE BETA SUBUNIT, ... (11 entities in total)
• 機能のキーワード: sarcosine oxidase, ligand complex, oxidoreductase
• 由来する生物種: Corynebacterium sp. U-96; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 183005.48

構造登録者

Suzuki, H.,Moriguchi, T.,Ida, K. (登録日: 2010-01-15, 公開日: 2010-08-25, 最終更新日: 2023-11-01)

主引用文献

Moriguchi, T.,Ida, K.,Hikima, T.,Ueno, G.,Yamamoto, M.,Suzuki, H.
Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96.
J.Biochem., 148:491-505, 2010

PubMed Abstract: We characterized the crystal structures of heterotetrameric sarcosine oxidase (SO) from Corynebacterium sp. U-96 complexed with methylthioacetate (MTA), pyrrole 2-carboxylate (PCA) and sulphite, and of sarcosine-reduced SO. SO comprises α-, β-, γ- and δ-subunits; FAD and FMN cofactors; and a large internal cavity. MTA and PCA are sandwiched between the re-face of the FAD isoalloxazine ring and the β-subunit C-terminal residues. Reduction of flavin cofactors shifts the β-subunit Ala1 towards the α-subunit Met55, forming a surface cavity at the oxygen-channel vestibule and rendering the β-subunit C-terminal residues mobile. We identified three channels connecting the cavity and the enzyme surface. Two of them exist in the inter-subunit space between α and β-subunits, and the substrate sarcosine seems to enter the active site through either of these channels and reaches the re-side of the FAD isoalloxazine ring by traversing the mobile β-subunit C-terminal residues. The third channel goes through the α-subunit and has a folinic acid-binding site, where the iminium intermediate is converted to Gly and either formaldehyde or, 5,10-methylenetetrahydrofolate. Oxygen molecules are probably located on the surface cavity and diffuse to the FMN isoalloxazine ring; the H(2)O(2) formed exits via the oxygen channel.

PubMed: 20675294
DOI: 10.1093/jb/mvq083

実験手法

X-RAY DIFFRACTION (2.2 Å)