3A51

Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound 25-hydroxyvitamin D3

3A51 の概要

• エントリーDOI: 10.2210/pdb3a51/pdb
• 関連するPDBエントリー: 3A4G 3A4H 3A4Z 3A50
• 分子名称: Vitamin D hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, 3-{2-[1-(5-HYDROXY-1,5-DIMETHYL-HEXYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-4-METHYLENE-CYCLOHEXANOL, ... (7 entities in total)
• 機能のキーワード: cytochrome p450, vitamin d3 hydroxylase, hemoprotein, monooxygenase, directed evolution, oxidoreductase
• 由来する生物種: Pseudonocardia autotrophica
• 細胞内の位置: Cytoplasm : C4B644
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 233863.02

構造登録者

Yasutake, Y.,Fujii, Y.,Cheon, W.K.,Arisawa, A.,Tamura, T. (登録日: 2009-07-24, 公開日: 2010-07-28, 最終更新日: 2023-11-01)

• 主引用文献: Yasutake, Y.,Fujii, Y.,Nishioka, T.,Cheon, W.K.,Arisawa, A.,Tamura, T.; Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase; J.Biol.Chem., 285:31193-31201, 2010; PubMed: 20667833; DOI: 10.1074/jbc.M110.147009

実験手法

X-RAY DIFFRACTION (2 Å)