3A4Z

Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution

Summary for 3A4Z

• Entry DOI: 10.2210/pdb3a4z/pdb
• Related: 3A4G 3A4H 3A50 3A51
• Descriptor: Vitamin D hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: cytochrome p450, vitamin d3 hydroxylase, hemoprotein, monooxygenase, directed evolution, oxidoreductase
• Biological source: Pseudonocardia autotrophica
• Cellular location: Cytoplasm : C4B644
• Total number of polymer chains: 5
• Total formula weight: 231800.79

Authors

Yasutake, Y.,Fujii, Y.,Cheon, W.K.,Arisawa, A.,Tamura, T. (deposition date: 2009-07-24, release date: 2010-07-28, Last modification date: 2023-11-01)

• Primary citation: Yasutake, Y.,Fujii, Y.,Nishioka, T.,Cheon, W.K.,Arisawa, A.,Tamura, T.; Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase; J.Biol.Chem., 285:31193-31201, 2010; PubMed: 20667833; DOI: 10.1074/jbc.M110.147009

Experimental method

X-RAY DIFFRACTION (2.2 Å)