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3A4Z

Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, D, E
(A, B, C, D, E)
Vitamin D hydroxylasepolymer41145587.95UniProt (C4B644)
Pfam (PF00067)
Pseudonocardia autotrophica
2F, M, N, R, U
(A, B, C, D, E)
PROTOPORPHYRIN IX CONTAINING FEnon-polymer616.55Chemie (HEM)
3G, H, I, O, S...
(A, C, D, E)
CALCIUM IONnon-polymer40.16Chemie (CA)
4J, P, Q, T, W...
(A, C, D, E)
ACETATE IONnon-polymer59.06Chemie (ACT)
5K, L
(A)
GLYCEROLnon-polymer92.12Chemie (GOL)
6AA, BA, CA, Y, Z
(C, D, E, A, B)
waterwater18.0814Chemie (HOH)
Sequence modifications
A, B, C, D, E: 1 - 403 (UniProt: C4B644)
PDBExternal DatabaseDetails
Arg 70Thr 70engineered mutation
Leu 156Val 156engineered mutation
Met 216Glu 216engineered mutation
Arg 384Glu 384engineered mutation
Leu 404-expression tag
Glu 405-expression tag
His 406-expression tag
His 407-expression tag
His 408-expression tag
His 409-expression tag
His 410-expression tag
His 411-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight227939.4
Non-Polymers*Number of molecules19
Total formula weight3861.4
All*Total formula weight231800.8
*Water molecules are not included.

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PDB entries from 2025-07-09

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