3TIE

Crystal structure of the vaccinia derived peptide A11R in complex with the murine MHC CLASS I H-2 KB

Summary for 3TIE

• Entry DOI: 10.2210/pdb3tie/pdb
• Related: 1FG2 1S7R 3TID
• Descriptor: H-2 class I histocompatibility antigen, K-B alpha chain, Beta-2-microglobulin, Vaccinia derived octamer peptide, ... (4 entities in total)
• Functional Keywords: antigen presentation, peptide binding, igg, mhc, immune system
• Biological source: Mus musculus (mouse); More
• Cellular location: Membrane; Single-pass type I membrane protein: P01901; Secreted : P61769
• Total number of polymer chains: 6
• Total formula weight: 91127.92

Authors

Nguyen, T.T.,Shen, Z.T.,Stern, L.J. (deposition date: 2011-08-20, release date: 2012-08-22, Last modification date: 2024-11-27)

Primary citation

Shen, Z.T.,Nguyen, T.T.,Daniels, K.A.,Welsh, R.M.,Stern, L.J.
Disparate epitopes mediating protective heterologous immunity to unrelated viruses share peptide-MHC structural features recognized by cross-reactive T cells.
J.Immunol., 191:5139-5152, 2013

PubMed Abstract: Closely related peptide epitopes can be recognized by the same T cells and contribute to the immune response against pathogens encoding those epitopes, but sometimes cross-reactive epitopes share little homology. The degree of structural homology required for such disparate ligands to be recognized by cross-reactive TCRs remains unclear. In this study, we examined the mechanistic basis for cross-reactive T cell responses between epitopes from unrelated and pathogenic viruses, lymphocytic choriomeningitis virus (LCMV) and vaccinia virus. Our results show that the LCMV cross-reactive T cell response toward vaccinia virus is dominated by a shared asparagine residue, together with other shared structural elements conserved in the crystal structures of K(b)-VV-A11R and K(b)-LCMV-gp34. Based on analysis of the crystal structures and the specificity determinants for the cross-reactive T cell response, we were able to manipulate the degree of cross-reactivity of the T cell response, and to predict and generate a LCMV cross-reactive response toward a variant of a null OVA-derived peptide. These results indicate that protective heterologous immune responses can occur for disparate epitopes from unrelated viruses.

PubMed: 24127554
DOI: 10.4049/jimmunol.1300852

Experimental method

X-RAY DIFFRACTION (2.25 Å)