3TIE
Crystal structure of the vaccinia derived peptide A11R in complex with the murine MHC CLASS I H-2 KB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-10-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.089 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.264, 84.710, 87.895 |
Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
Resolution | 43.507 - 2.250 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.21930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.178 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 47727 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 8K, Cacodylate, Magnesium Acetate, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |