3SH8

Crystal structure of fluorophore-labeled beta-lactamase PenP in complex with cephaloridine

Replaces:  3KGO

Summary for 3SH8

• Entry DOI: 10.2210/pdb3sh8/pdb
• Related: 3SH7 3SH9
• Descriptor: Beta-lactamase, 5-METHYL-2-[2-OXO-1-(2-THIOPHEN-2-YL-ACETYLAMINO)-ETHYL]-3,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID (3 entities in total)
• Functional Keywords: beta-lactamase, fluorophore, cephaloridine, hydrolase-antibiotic complex, hydrolase/antibiotic
• Biological source: Bacillus licheniformis
• Cellular location: Cell membrane; Lipid-anchor (Probable): P00808
• Total number of polymer chains: 2
• Total formula weight: 59976.16

Authors

Wong, W.-T.,Zhao, Y.-X.,Leung, Y.-C. (deposition date: 2011-06-16, release date: 2011-07-27, Last modification date: 2024-03-20)

• Primary citation: Wong, W.-T.,Chan, K.-C.,So, P.-K.,Yap, H.-K.,Chung, W.-H.,Leung, Y.-C.,Wong, K.-Y.,Zhao, Y.-X.; Increased structural flexibility at the active site of a fluorophore-conjugated beta-lactamase distinctively impacts its binding toward diverse cephalosporin antibiotics; J.Biol.Chem., 286:31771-31780, 2011; PubMed: 21705325; DOI: 10.1074/jbc.M110.198895

Experimental method

X-RAY DIFFRACTION (2 Å)