3NVQ
Molecular mechanism of guidance cue recognition
Summary for 3NVQ
| Entry DOI | 10.2210/pdb3nvq/pdb |
| Related | 3NVX 3nvn |
| Descriptor | Semaphorin-7A, Plexin-C1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total) |
| Functional Keywords | beta-propeller, signaling, signaling protein-protein binding complex, signaling protein/protein binding |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 4 |
| Total formula weight | 242651.05 |
| Authors | |
| Primary citation | Liu, H.,Juo, Z.S.,Shim, A.H.,Focia, P.J.,Chen, X.,Garcia, K.C.,He, X. Structural Basis of Semaphorin-Plexin Recognition and Viral Mimicry from Sema7A and A39R Complexes with PlexinC1. Cell(Cambridge,Mass.), 142:749-761, 2010 Cited by PubMed Abstract: Repulsive signaling by Semaphorins and Plexins is crucial for the development and homeostasis of the nervous, immune, and cardiovascular systems. Sema7A acts as both an immune and a neural Semaphorin through PlexinC1, and A39R is a Sema7A mimic secreted by smallpox virus. We report the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1. Both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers, in which the Semaphorin and PlexinC1 beta propellers interact in an edge-on, orthogonal orientation. Both binding interfaces are dominated by the insertion of the Semaphorin's 4c-4d loop into a deep groove in blade 3 of the PlexinC1 propeller. A39R appears to achieve Sema7A mimicry by preserving key Plexin-binding determinants seen in the mammalian Sema7A complex that have evolved to achieve higher affinity binding to the host-derived PlexinC1. The complex structures support a conserved Semaphorin-Plexin recognition mode and suggest that Plexins are activated by dimerization. PubMed: 20727575DOI: 10.1016/j.cell.2010.07.040 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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