3JYT

K65R mutant HIV-1 reverse transcriptase cross-linked to DS-DNA and complexed with DATP as the incoming nucleotide substrate

Summary for 3JYT

• Entry DOI: 10.2210/pdb3jyt/pdb
• Related: 1RTD 1T05 3JSM
• Descriptor: Reverse transcriptase/ribonuclease H, p51 RT, DNA (5'-D(*A*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3'), ... (7 entities in total)
• Functional Keywords: hiv-1 reverse transcriptase, tenofovir, rt-dna complex, transferase-dna complex, drug resistance mutation, aids, dna recombination, dna-directed dna polymerase, rnase h, hydrolase, lipoprotein, magnesium, membrane, metal-binding, multifunctional enzyme, nucleotidyltransferase, rna-directed dna polymerase transferase, transferase-dna complex complex, transferase/dna complex
• Biological source: Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1); More
• Cellular location: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P03366 P03366
• Total number of polymer chains: 4
• Total formula weight: 130962.86

Authors

Das, K.,Arnold, E. (deposition date: 2009-09-22, release date: 2009-10-06, Last modification date: 2023-09-06)

• Primary citation: Das, K.,Bandwar, R.P.,White, K.L.,Feng, J.Y.,Sarafianos, S.G.,Tuske, S.,Tu, X.,Clark, A.D.,Boyer, P.L.,Hou, X.,Gaffney, B.L.,Jones, R.A.,Miller, M.D.,Hughes, S.H.,Arnold, E.; Structural basis for the role of the K65r mutation in HIV-1 reverse transcriptase polymerization, excision antagonism, and tenofovir resistance.; J.Biol.Chem., 284:35092-35100, 2009; PubMed: 19812032; DOI: 10.1074/jbc.M109.022525

Experimental method

X-RAY DIFFRACTION (3.3 Å)