3JBR

Cryo-EM structure of the rabbit voltage-gated calcium channel Cav1.1 complex at 4.2 angstrom

Summary for 3JBR

• Entry DOI: 10.2210/pdb3jbr/pdb
• EMDB information: 6475 6476
• Descriptor: Voltage-dependent L-type calcium channel subunit alpha-1S, Voltage-dependent L-type calcium channel subunit beta-2, Voltage-dependent calcium channel gamma-1 subunit, ... (7 entities in total)
• Functional Keywords: membrane protein, voltage-gated calcium channel
• Biological source: Rattus norvegicus (rat); More
• Total number of polymer chains: 4
• Total formula weight: 394532.24

Authors

Wu, J.P.,Yan, Z.,Yan, N. (deposition date: 2015-09-29, release date: 2015-12-30, Last modification date: 2024-10-23)

Primary citation

Wu, J.P.,Yan, Z.,Li, Z.Q.,Yan, C.G.,Lu, S.,Dong, M.Q.,Yan, N.
Structure of the voltage-gated calcium channel Cav1.1 complex
Science, 350:aad2395-aad2395, 2015

PubMed Abstract: The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. The Ca(v)1.1 complex consists of the pore-forming subunit α1 and auxiliary subunits α2δ, β, and γ. We report the structure of the rabbit Ca(v)1.1 complex determined by single-particle cryo-electron microscopy. The four homologous repeats of the α1 subunit are arranged clockwise in the extracellular view. The γ subunit, whose structure resembles claudins, interacts with the voltage-sensing domain of repeat IV (VSD(IV)), whereas the cytosolic β subunit is located adjacent to VSD(II) of α1. The α2 subunit interacts with the extracellular loops of repeats I to III through its VWA and Cache1 domains. The structure reveals the architecture of a prototypical eukaryotic Ca(v) channel and provides a framework for understanding the function and disease mechanisms of Ca(v) and Na(v) channels.

PubMed: 26680202
DOI: 10.1126/science.aad2395

Experimental method

ELECTRON MICROSCOPY (4.2 Å)