3I5U

Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosylmethionine (SAM) and 2-hydroxy-5-methyl naphthoic acid (MNA)

Summary for 3I5U

Entry DOI	10.2210/pdb3i5u/pdb
Related	3I53 3I58 3I64
Descriptor	O-methyltransferase, S-ADENOSYLMETHIONINE, 2-hydroxy-5-methyl naphthoic acid, ... (5 entities in total)
Functional Keywords	co-complex, rossmann-like fold, methyltransferase, transferase
Biological source	Streptomyces carzinostaticus subsp. neocarzinostaticus
Total number of polymer chains	2
Total formula weight	70651.68
Authors	Cooke, H.A.,Bruner, S.D. (deposition date: 2009-07-06, release date: 2009-09-01, Last modification date: 2023-09-06)
Primary citation	Cooke, H.A.,Guenther, E.L.,Luo, Y.,Shen, B.,Bruner, S.D. Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. Biochemistry, 48:9590-9598, 2009 Cited by PubMed: 19702337 DOI: 10.1021/bi901257q PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation